BPC-157 Reconstitution Calculator: Concentration and Units

A bpc 157 reconstitution calculator converts two inputs into a concentration: the milligrams of peptide in the vial and the milliliters of bacteriostatic water added. The output tells you how many micrograms are contained in each unit of volume you draw. This is arithmetic. It is not dosing guidance for any species. We built this tool because the concentration math is simple but easy to get wrong when switching between milligrams, micrograms and syringe units. BPC-157 is not approved for human use by the FDA or any regulatory body.

BPC-157 Reconstitution Calculator

How the Math Works

Concentration Formula

The formula is: mg/ml = mg of peptide / ml of water. A 5 mg vial reconstituted with 2 ml of bacteriostatic water produces a concentration of 2.5 mg/ml. That is 2500 mcg/ml. Every 0.1 ml drawn from this solution contains 250 mcg. The total peptide in the vial does not change regardless of how much water is added. More water means a lower concentration per unit volume.

Reading a U-100 Insulin Syringe

A standard U-100 insulin syringe holds 1 ml total. The barrel is marked in 100 units. Each unit equals 0.01 ml. Ten units equals 0.1 ml. This is important because peptide concentrations are typically expressed per 0.1 ml draw. If your solution is 2.5 mg/ml, drawing to the 10-unit line gives you 250 mcg. Drawing to the 5-unit line gives you 125 mcg. The syringe measures volume. The concentration determines how much peptide that volume contains.

Step-by-Step Concentration Arithmetic

5mg Vial Examples

Start with a 5 mg vial. Add 1 ml of bacteriostatic water. Divide 5 by 1. The concentration is 5.0 mg/ml. In micrograms, that is 5000 mcg/ml. Each 0.1 ml (10 units on a U-100 syringe) contains 500 mcg.

Same vial, 2 ml of water. 5 divided by 2 equals 2.5 mg/ml. That is 2500 mcg/ml. Each 0.1 ml contains 250 mcg. The total peptide remains 5 mg. Only the concentration per draw changes.

Same vial, 2.5 ml of water. 5 divided by 2.5 equals 2.0 mg/ml. Each 0.1 ml draw contains 200 mcg. Higher water volumes produce lower concentrations and require larger draw volumes for the same peptide amount per draw.

10mg Vial Examples

A 10 mg vial with 2 ml of water. 10 divided by 2 equals 5.0 mg/ml. Each 0.1 ml contains 500 mcg. This is the same concentration as a 5 mg vial with 1 ml of water. The difference is that the 10 mg vial contains twice the total peptide, so you get twice as many draws at the same concentration.

10 mg with 5 ml of water. 10 divided by 5 equals 2.0 mg/ml. Each 0.1 ml contains 200 mcg. We find that 5 ml is the upper practical limit because larger water volumes dilute the solution to a point where draw volumes become inconvenient.

10 mg with 1 ml of water. This produces 10.0 mg/ml or 1000 mcg per 0.1 ml. It is a highly concentrated solution. Precision matters more at this concentration because small volume errors translate to large peptide differences.

Storage After Reconstitution

Refrigeration Requirements

Reconstituted BPC-157 must be stored at 2-8 degrees Celsius. This is standard refrigerator temperature. The peptide degrades faster at room temperature. Light exposure accelerates degradation. We recommend wrapping the vial in foil or storing it in a dark section of the refrigerator. Do not freeze a reconstituted solution. Freezing can damage peptide structure through ice crystal formation.

Shelf Life of Reconstituted Peptide

When reconstituted with bacteriostatic water containing 0.9% benzyl alcohol, the solution remains usable for 28-30 days at refrigerator temperature. Bacteriostatic water inhibits microbial growth but does not sterilize the solution. The 28-day limit is a conservative guideline used across peptide vendors. If the solution turns cloudy, develops particles, or changes color, discard it. Sterile water lacks a preservative and should only be used if the entire contents will be drawn in a single session. For the BPC-157 injection dosage chart, see our separate reference page.